Today we are introducing the product of amino acid analyzer solvent safety bottle cap. We know that liquid chromatograph is an instrument for separation and analysis of solution samples. The chromatograph is mainly composed of sampling system, infusion system, separation system, detection system and data Processing system and other parts. It is widely used in the detection, analysis and data recording of chemical products, and can be used for the analysis and detection of liquids or solid samples dissolved in liquids.

Liquid chromatography

HPLC mobile phase safety bottle cap

The amino acid analyzer refers to a method used to determine the amino acid composition or content of proteins, peptides and other pharmaceutical preparations. Before amino acid analysis, proteins and peptides must be hydrolyzed into individual amino acids. It is an ion exchange chromatograph based on cation exchange column separation, post-column ninhydrin derivatization, and photometric determination. The amino acid analyzer consists of a chromatographic column, autosampler, detector, data recording and processing system.

The basic principle of the amino acid analyzer is that the mobile phase (buffer solution) pushes the amino acid mixture through the chromatographic column equipped with cation exchange resin, and each amino acid exchanges ion with the exchange group in the resin. When eluted with different pH buffer solutions When the amino acid mixture is separated due to the difference in exchange capacity, the separated single amino acid component reacts with the ninhydrin reagent to generate a purple compound or a yellow compound, and the absorbance at 570nm and 440rnn is detected by a visible light detector.

The basic structure of the instrument is similar to that of ordinary HPLC, but the details are optimized for amino acid analysis (such as nitrogen protection, inert pipeline, online degassing, elution gradient and column temperature gradient control, etc.).

Amino acid analyzer solvent safety bottle cap

Liquid chromatographs use organic solvents as mobile phases. Now the usual practice is to directly drill holes on the mobile phase bottle caps to deepen the pipeline into the solvent. In order to prevent the solvent from volatilizing, it is supplemented with a sealing film to cover it. However, this traditional method There are three problems with this approach:

1. Cannot be completely sealed

If the mouth of the bottle is sealed with a sealing film to block air circulation, a closed negative pressure vacuum environment will be formed in the solvent bottle during solvent injection, and the solvent cannot be extracted.

2. Volatilization of mobile phase pollutes the environment

Since the mouth of the bottle cannot be completely sealed with a sealing film, the volatile mobile phase will evaporate from the gap in the bottle cap into the indoor air, resulting in a great smell of indoor solvents, causing environmental pollution and serious damage to the health of the staff.

3. Dust enters the contaminated mobile phase

Since the mouth of the bottle cannot be completely sealed with a sealing film, when the chromatograph is extracting solvent, impurities such as dust in the air will enter the mobile phase bottle along with the air, causing solvent pollution and seriously affecting the analysis results.

The liquid chromatography solvent safety bottle cap product produced by Beijing Aowei Instrument Co., Ltd. can efficiently filter the dust in the air when the chromatograph extracts the solvent, prevent the mobile phase from contaminating the mobile phase, ensure the accuracy of the analysis result, and achieve completeness when the chromatographic instrument stops working. It is sealed to prevent the mobile phase from volatilizing outward through the air inlet and protect the laboratory environment from pollution.

HPLC mobile phase safety bottle cap

Schematic diagram of HPLC mobile phase safety bottle cap

Main features of Beijing Aowei Instrument Co., Ltd. SE series solvent safety bottle caps:

Microporous membrane-high efficiency filtration

The SE series solvent safety bottle cap has a built-in PTFE microporous filter membrane. When the chromatograph extracts solvent, the air entering the mobile phase bottle is efficiently filtered to prevent dust from polluting the solvent and ensure the accuracy of the analysis results;

One-way seal-prevent volatilization

SE series solvent safety bottle caps use organic solvent-resistant high-sensitivity one-way safety valve. When the chromatographic instrument stops working, the safety valve is automatically closed and sealed to prevent the solvent in the bottle from evaporating outward, protect the laboratory environment and the health of the experimenters;

Life indicator-due reminder

When the SE series solvent safety bottle caps are used, press the time start button on the safety valve, and the life indicator label will record the use time in the form of a red progress bar, reminding users to replace the safety valve on schedule;

Plug-in installation-easy to replace

SE series solvent safety bottle cap adopts quick plug-in installation design, which can be replaced by users;

Multiple types-wide compatibility

SE series solvent safety bottle caps have SE45, SE45, SE38 type, compatible with most solvent bottle specifications, and can be used with HPLC of internationally renowned brands such as Agilent, Thermo Fisher, Waters, Shimadzu, PerkinElmer, etc. Perfectly carry the app.

Model Appearance Suitable for bottle mouth specifications

SE45 type SE45 type HPLC mobile phase safety bottle cap GL45 standard bottle mouth

SE40 type SE40 type HPLC mobile phase safety bottle cap GL40 standard bottle mouth

SE38 type SE38 type HPLC mobile phase safety bottle cap GL38 standard bottle mouth

Green laboratory solvent safety bottle cap